An investigation on injectable composites fabricated by 45S5 bioactive glass and gum tragacanth: Rheological properties and in vitro behavior

Authors

Department of Nanotechnology & Advanced Materials, Materials and Energy Research Center, Karaj, Iran

Abstract

The injectable composites were formulated from melt-derived 45S5 bioactive glass powder and gum tragacanth. The effect of the tragacanth (2 and 4 w/v%) and powder to liquid ratio (P/L= 1.5 to 2.5) on rheological properties, injectability, degradation, swelling, and bioactivity the composites were studied. With increasing the P/L ratio and tragacanth concentration, the force required for injection of the composites is increased. However, the formulated composites show maximum injection force of 15 N, which seems to be appropriate for surgical purposes. The formulated composites indicate positive thixotropic behavior, whereas increasing tragacanth from 2% to 4% lead to deteriorating its behavior. Moreover, thecomposites formulated by 2% tragacanth show much more resistance against degradation and swelling. The bioactivity analyze confirms the formation of flake-like apatite nanostructures on the surface of nanocomposites in initial days of immersion into the SBF solution.

Keywords

Main Subjects


1. Mano, J.F., Sousa, R.A., Boesel, L.F., Neves, N.M. and Reis, R.L., “Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments”, Composites Science and Technology Vol. 64, (2004), 789-817.
2. González, C., Vilatela, J.J., Molina-Aldareguía, J.M., Lopes, C.S. and Llorca, J., “Structural composites for multifunctional applications: Current challenges and future trends”, Progress in Materials Science, Vol. 89, (2017), 194-251.
3. Venkatesan, J., Bhatnagar, I., Manivasagan, P., Kang, K.H. and Kim, S.K., “Alginate composites for bone tissue engineering: A review”, International Journal of Biological Macromolecules, Vol. 72, (2015), 269-281.
4. Baino, F., Fiorilli, S. and Vitale-Brovarone, C., “Bioactive glassbased materials with hierarchical porosity for medical applications: Review of recent advances”, Acta Biomaterialia, Vol. 42, (2016), 18-32.
5. Miguez-Pacheco, V., Hench, L.L. and Boccaccini, A.R., “Bioactive glasses beyond bone and teeth: Emerging applications in contact with soft tissues”, Acta Biomaterialia, Vol. 13, (2015), 1-15.
6. Jones, J.R., “Review of bioactive glass: From Hench to hybrids”, Acta Biomaterialia, Vol. 23, (2015) s53-s82.
7. Park, S.B., Lih, E., Park, K.S., Joung, Y.K. and Han, D.K., “Biopolymer-based functional composites for medical applications, Progress in Polymer Science, Vol. 68, (2017), 77- 105.
8. Rao, S.H., Harini, B., Shadamarshan, R.P.K., Balagangadharan, K. and Selvamurugan, N.,“ Natural and synthetic polymers/bioceramics/bioactive compounds-mediated cell signalling in bone tissue engineering”, International Journal of Biological Macromolecules, Vol. 110, (2018), 88-96.
9. Zhao, W., Jin, X., Cong, Y. Liu, Y. and Fu, J., “Degradable natural polymer hydrogels for articular cartilage tissue engineering”, Journal of Chemical Technology and Biotechnology, Vol. 88, (2013), 327-339.
10. Gyles, D.A., Castro, L.D., Carréra Silva, J.O. and Ribeiro-Costa, R.M., “A review of the designs and prominent biomedical advances of natural and synthetic hydrogel formulations”, European Polymer Journal, Vol. 88, (2017), 373-392.
11. Ghayempour, S. and Montazer, M.A, “modified microemulsion method for fabrication of hydrogel Tragacanth nanofibers”, International Journal of Biological Macromolecules, Vol. 115, (2018), 317-323.
12. Rahmani, Z., Sahraei, R. and Ghaemy, M., “Preparation of spherical porous hydrogel beads based on ion-crosslinked gum tragacanth and graphene oxide: Study of drug delivery behavior”, Carbohydrate Polymers, Vol. 194, (2018), 34-42.
13. Sohrabi, M., Hesaraki, S., and Kazemzadeh, A., “ Injectable Bioactive Glass/Polysaccharide Polymers Nanocomposites for Bone Substitution”, Key Enginnering and Materials, Vol. 614, (2014), 41-46.
14. Tulyaganov, D., Abdukayumov, K., Ruzimuradov, O., Hojamberdiev, M., Ionescu, E., and Riedel, R., “Effect of alumina incorporation on the surface mineralization and degradation of a bioactive glass (CaO-MgO-SiO2-Na2O-P2O5- CaF2)-glycerol paste”, Materials, Vol. 10, (2017), 1324.
15. Aho, A.J., Tirri, T., Kukkonen, J., Strandberg, N., Rich, J., Seppälä, J., and Yli-Urpo, A., “Injectable bioactive glass/biodegradable polymer composite for bone and cartilage reconstruction: Concept and experimental outcome with thermoplastic composites of poly(ε-caprolactone-co-D,Llactide) and bioactive glass S53P4”, Journal of Materials Science: Materials in Medicine, Vol. 15, (2004), 1165–1173.
16. Borhan, S., Hesaraki, S., Behnamghader, A.A., and Ghasemi, E., “Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites”, Journal of Materials Science: Materials in Medicine, Vol. 27, (2016), 137.
17. Nwe, N., Furuike, T., and Tamura, H., “The mechanical and biological propertiesof chitosan scaffolds for tissue regeneration templates are significantlyenhanced by chitosan from Gongronellabutleri”, Materials, Vol. 2, (2009), 374–398.
18. Shamsi, M., Karimi, M., Ghollasi, M., Nezafati, N., Shahrousvand, M., Kamali, M., and Salimi, A., “In vitro proliferation and differentiation of human bone marrow mesenchymal stem cells into osteoblasts on nanocomposite scaffolds based on bioactive glass (64SiO2-31CaO-5P2O5)-polyl- lactic acid nanofibers fabricated by electrospinning method”, Materials Science and Engineering C, Vol. 78, (2017), 114- 123.
19. Karimi, M., Hesaraki, S., Alizadeh, M., and Kazemzadeh, A., “A facile and sustainable method based on deep eutectic solvents toward synthesis of amorphous calcium phosphate nanoparticles: The effect of using various solvents and precursors on physical characteristics”, Journal of Non-Crystalline Solids, Vol. 443 (2016) 59–64.
20. Karimi, M., Hesaraki, S., Alizadeh, M. and Kazemzadeh, A. , “Synthesis of calcium phosphate nanoparticles in deep-eutectic choline chloride–urea medium: Investigating the role of synthesis temperature on phase characteristics and physical properties”, Ceramics International, Vol. 42, (2016), 2780–
2788.
21. Koike, N., Ikuno, T., Okubo, T, and Shimojima, A., “Synthesis of monodisperseorganosilica nanoparticles with hollow interiors and porous shells using silica nanospheres as templates”, Chemical Communications, Vol. 49, (2013), 4998.
22. Faure, J., Drevet, R., Lemelle, A., Ben Jaber, N., Tara, A., Btaouri, H.E., and Benhayoune, H., “A new sol-gel synthesis of 45S5 bioactive glass using an organic acid as catalyst”, Materials Science and Engineering C, Vol. 47, (2015), 407- 412.
23. Sohrabi, M., Hesaraki, S., Kazemzadeh, A., and Alizadeh, M., “Development of injectable biocomposites from hyaluronic acid and bioactive glass nano-particles obtained from different sol– gel routes”, Materials Science and Engineering C, Vol. 33, (2013), 3730-3744.
24. Borhan, S., Hesaraki, S., Behanamghader, A.A., and Ghasemi, E., “Injectable bone paste biocomposite based on melt-derived bioactive glass and sodium alginate natural polymer”, Journal of the Australian Ceramic Society, Vol. 51, (2015), 99-108.
25. Habib, M., Baroud, G., Gitzhofer, F., and Bohner, M., “Mechanisms underlying the limited injectability of hydraulic calcium phosphate paste”, Acta Biomaterialia, Vol. 4, (2008), 1465-1471.
26. Gabbai-Armelin, P.R., Cardoso, D.A., Zanotto, E.D., Peitl, O., Leeuwenburgh, S.C.G., Jansen, J.A., Renno, A.C.M., and van den Beucken, J.J.J.P., “Injectable composites based on biosilicate® and alginate: handling and in vitro characterization”, RSC Advances, Vol. 4, (2014), 45778-45785.
27. Karimi, M., Hesaraki, S., and Nezafati, N., “In vitro biodegradability–bioactivity-biocompatibility and antibacterial properties of SrF2 nanoparticles synthesized by one-pot and ecofriendly method based on ternary strontium chloride-choline chloride-water deep eutectic system”, Ceramics International, Vol. 44, (2018), 12877–12885.
28. Hill, R., “An alternative view of the degradation of bioglass”, Journal of Materials Science Letters, Vol. 15, (1996), 1122- 1125.
29. Karimi, M., Hesaraki, S., Alizadeh, M. and Kazemzadeh, A., “Time and temperature mediated evolution of CDHA from ACP nanoparticles in deep eutectic solvents: Kinetic and thermodynamic considerations”, Materials and Design, Vol. 122 (2017), 1–10.
30. Karimi, M., Jodaei, A., Sadeghinik, A., Rastegar Ramsheh, M., Mohammadi Hafshejani, T., Shamsi, M., Orand, F. and Lotfi, F., “Deep eutectic choline chloride-calcium chloride as all-inone system for sustainable and one-step synthesis of bioactive fluorapatite nanoparticles”, Journal of Fluorine Chemistry, Vol. 204, (2017), 76–83.